Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment

Carlota Rubio-Perez ^# 1, Ester Planas-Rigol ^# 1, Juan L Trincado ^# 2 3, Ester Bonfill-Teixidor ¹, Alexandra Arias ¹, Domenica Marchese ², Catia Moutinho ², Garazi Serna ¹, Leire Pedrosa ⁴, Raffaella Iurlaro ¹, Francisco Martínez-Ricarte ^5 6, Laura Escudero ¹, Esteban Cordero ^5 6, Marta Cicuendez ^5 6, Sara Ruiz ², Genís Parra ², Paolo Nuciforo ¹, Josep Gonzalez ⁴, Estela Pineda ⁴, Juan Sahuquillo ^5 6, Josep Tabernero ^1 6 7, Holger Heyn ^8 9, Joan Seoane ^{10 11 12 13}Affiliations expand

• PMID: 33686071
• PMCID: PMC7940606
• DOI: 10.1038/s41467-021-21789-x

Free PMC article

Abstract

Brain metastases are the most common tumor of the brain with a dismal prognosis. A fraction of patients with brain metastasis benefit from treatment with immune checkpoint inhibitors (ICI) and the degree and phenotype of the immune cell infiltration has been used to predict response to ICI. However, the anatomical location of brain lesions limits access to tumor material to characterize the immune phenotype. Here, we characterize immune cells present in brain lesions and matched cerebrospinal fluid (CSF) using single-cell RNA sequencing combined with T cell receptor genotyping. Tumor immune infiltration and specifically CD8⁺ T cell infiltration can be discerned through the analysis of the CSF. Consistently, identical T cell receptor clonotypes are detected in brain lesions and CSF, confirming cell exchange between these compartments. The analysis of immune cells of the CSF can provide a non-invasive alternative to predict the response to ICI, as well as identify the T cell receptor clonotypes present in brain metastasis.

Editor do blog: Julio Pereira – Neurocirugião – São Paulo CRM/SP 163.113 Site para Consulta: https://www.julio-pereira.com/ Consultório: (11)99503-8838 (WhatsApp) / (11)4200-2300 Atuando no Hospital Sírio-Libanês e na BP – A Beneficência Portuguesa de São Paulo.

CONFIRA NOSSOS SITES:
Cirurgia de Coluna
Tumor Cerebral
Aneurisma Cerebral
Radiocirurgia
Neuro Cancer
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